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Practical Courses

COURSE CATALOGE 2017/2018


Application Winterterm

Application for all practical courses and seminars from 9.10. to 13.10.2017 via LSF
Preliminary meeting, 16.10.2017, 5.30pm, lecture hall B00.019, Gr. Hörsaal.

Application Summerterm

Application for all practical courses and seminars march 2018 via LSF

Practical Course Lecturer Seminar NoteECTSRecomended LectureSemester Resgistration 
Tumorepiegentics  Meilinger incl.  2 Parallels 6 Epigenetics, The Good The Bad The Ugly SS -
DNA Demethylation Meilinger incl. 6 Epigenetics, Methods in molecular biology SS -
Bioimaging  Harz  incl. 6 Methods in molecular biology  SS/WS LSF (WS)
Embryonic Stem Cells Meilinger, Bultmann incl. 2 Parallels 6 Epigenetics, The Good The Bad The Ugly WS LSF
Biological Databases Bultmann incl. 6 Methods in molecular biology WS LSF
The Essentials in Molecular Biology Meilinger - 1st Semster Master Students only, 2 Parallels 3 Methods in molecular biology WS LSF


COURSE CONTENT & DATES 

Practical Course: Tumorepigenetics (incl. Seminar)

  • 20.06.-30.06.2017 (group 1), 10:00-18:00, D00.021/D00.017
  • 10.07.-21.08.2017 (group 2), 10:00-18:00, D00.021/D00.017

Prof. Dr. Heinrich Leonhardt, Dr. Daniela Meilinger

Content: Aim of this course is to understand how epigenetic changes can favour tumorigenesis. Students will investigate the DNA methylation status of different tumorsuppressor genes in various cancer cell lines. In the weeks leading up to the course, each student pair will create a COBRA (Combined Bisulfite Restriction Analysis) assay for a selected tumorsuppressor gene, including the design of specific primer pairs that will be tested within the course.

Skills Taught: in silico primer design, COBRA assays design, PCR establishment, gradient PCR, genomic DNA extraction, bisulfite treatment, inhibitor testing, Immunostaining, fluorescence microscopy

Recommended Lectures: Epigenetics (SS), The Good The Bad & The Ugly (SS)

 

Practical Course: DNA Demethylation (incl. Seminar)

  • 24.04.-05.05.2017, 10:00-18:00, D00.021/D00.017

Prof. Dr. Heinrich Leonhardt, Dr. Daniela Meilinger

Content: This course aims to deepen the knowledge about DNA methylation, DNA modifications and especially how DNA demethylation is achieved in mammalian cells. Students will investigate the connection of TET enzymes, Base Excision Repair proteins and the Citric Acid Cycle using Co- Immunoprecipitation assays, an F3H Assay (Fluorescence 3-Hybrid Assay), immunostaining in embryonic stem cells and an uniquely designed APEX assay.

Skills Taught: Transfection of mammalian cells, Western Blot, Co-Immunoprecipitation, Immunostaining, fluorescence microscopy, F3H Assay, embryonic stem cell culture, APEX Assay (modified EMSA)

Recommended Lectures: Epigenetics (SS), Methods in Molecular Biology (WS)


Practical Course: BioImaging (incl. Seminar)

  • 24.04.2017, 17:30-18:30, Kl. Biologie 2
  • 25.07.-04.08.2017, 10:00-18:00, D00.021
  • 25.07.-06.08.2017, 10:00-18:00, D00.017

Prof. Dr. Heinrich Leonhardt, Dr. Hartmann Harz

 

Practical Course: Embryonic Stem Cells (incl. Seminar)

  • 20.06.-30.06.2017 (group 1), 10:00-18:00, D00.021/D00.017
  • 10.07.-21.08.2017 (group 2), 10:00-18:00, D00.021/D00.017

Prof. Dr. Heinrich Leonhardt, Dr. Daniela Meilinger

Content: This course teaches how to culture and handle mouse embryonic stem cells and moreover deepens the fundamental understanding of DNA methylation processes in mammalian cells. During the course, a rescue assay of knockout cell lines will be performed as well as a differentiation assay where ES cells will be differentiated into epiblast cells.

Skills Taught: ES cell culture, FACS sorting, genomic DNA extraction, bisulfite treatment, pyrosequencing, differentiation assay, Immunostaining, fluorescence microscopy FRAP, confocal microscopy, image analysis

Recommended Lectures: Epigenetics (SS), Methods in Molecular Biology (WS)


Practical Course: Biological Databases (incl. Seminar)

  • 20.06.-30.06.2017 (group 1), 10:00-18:00, D00.021/D00.017

Prof. Dr. Heinrich Leonhardt, Dr. Sebsatian Bultmann

Content:


Practical Course: The Essentials in molecular biology 

  • 20.06.-30.06.2017 (group 1), 10:00-18:00, D00.021/D00.017
  • 10.07.-21.08.2017 (group 2), 10:00-18:00, D00.021/D00.017

Prof. Dr. Heinrich Leonhardt, Dr. Daniela Meilinger

Content: This course is especially recommended for 1st semester Master Students, as it teaches essential and basic methods in molecular and cellular biology in-depth. Cloning techniques will be explored to generate a GFP Fusion protein including various mutants in order to investigate protein domain functions in vivo. After having successfully cloned different constructs, a Co-Immunoprecipitation will be performed to check for potential protein-protein interactions in mammalian cells. Finally, cells will be transfected with these constructs to visualize localisation in mammalian cells using fluorescence microscopy.

Skills Taught: Cloning to generate a GFP Fusion Protein, primer design, PCR, vector construction, ligation, transformation, "Mini-Prep" and "Maxi-Prep", Transfection of mammalian cells, Western Blot, Co-Immunoprecipitation, Immunostaining, fluorescence microscopy

Recommended Lectures: Methods in Molecular Biology (WS)