Human Biology & BioImaging
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Practical Courses

COURSE CATALOGE 2017/2018


Application Winterterm

Application for all practical courses and seminars only via LSF
After the "Selection Day" you will be contacted for a Preliminary meeting,

Practical Course Lecturer Seminar NoteECTSRecomended LectureSemester Registration 
Tumorepiegentics  Meilinger incl.  MCB  6 Epigenetics, The Good The Bad The Ugly SS -
Embryonic Stem Cells  & Epigenetics  Meilinger incl. MHB 6 Epigenetics, Methods in molecular biology SS -
Bioimaging  Harz  incl. 6 Methods in molecular biology  SS/WS LSF (WS)
Embryonic Stem Cells Meilinger, Bultmann incl. MCB 6 Epigenetics, The Good The Bad The Ugly WS LSF
Biological Databases Bultmann incl. MHB, MCB 6 Methods in molecular biology WS LSF
Molecular Oncology  Meilinger - MCB 6 The Good The Bad The Ugly WS LSF


COURSE CONTENT & DATES 

Practical Course: Tumorepigenetics (incl. Seminar)

  • 20.06.-30.06.2017 (group 1), 10:00-18:00, D00.021/D00.017

Prof. Dr. Heinrich Leonhardt, Dr. Daniela Meilinger

Content: Aim of this course is to understand how epigenetic changes can favour tumorigenesis. Students will investigate the DNA methylation status of different tumorsuppressor genes in various cancer cell lines. In the weeks leading up to the course, each student pair will create a COBRA (Combined Bisulfite Restriction Analysis) assay for a selected tumorsuppressor gene, including the design of specific primer pairs that will be tested within the course.

Skills Taught: in silico primer design, COBRA assays design, PCR establishment, gradient PCR, genomic DNA extraction, bisulfite treatment, inhibitor testing, Immunostaining, fluorescence microscopy

Recommended Lectures: Epigenetics (SS), The Good The Bad & The Ugly (SS)

 

Embryonic Stem Cells & Epigenetics (incl. Seminar)

Prof. Dr. Heinrich Leonhardt, Dr. Daniela Meilinger

Content: Main focus of this module is to understand the biology of ES cells, including pluripotency and their ability to differentiate into the 3 germ layers. Moreover, ES cells are on the only cells that can tolerate a complete loss of methylation, providing a perfect too to study epigenetic events, that naturally happen during embryogenesis. Leading up to the practical course, Students will work in teams and design their own experiments together with the course instructors. Experiments will be discussed and presented in a seminar (3 ECTS). After validation of these presented and suggested experiments, the students will practically perform these designed experiments within the 3 weeks of the course (3 ECTS). Students will learn how design experiments based on current literature, how to validate feasibly and how to actually perform them in the lab. Students can apply theoretical and practical knowledge to approach biological questions in independent work. Students will obtain skills for future lab work in the field of epigenetics and embryonic stem cells, in particular in preparation for their lab rotations and master’s thesis.

Skills Taught: Project Design, Assay Design, Team Work, emrbyonic stem cell culture, COBRA assays design, PCR establishment, gradient PCR, genomic DNA extraction, bisulfite treatment, inhibitor testing, Immunostaining, fluorescence microscopy

Recommended Lectures: Epigenetics (SS), Methods in Molecular Biology (WS)


Practical Course: BioImaging (incl. Seminar)

  • 25.07.-04.08.2017, 10:00-18:00, D00.021
  • 25.07.-06.08.2017, 10:00-18:00, D00.017

Prof. Dr. Heinrich Leonhardt, Dr. Hartmann Harz

 

Practical Course: Embryonic Stem Cells (incl. Seminar)

  • 20.06.-30.06.2017 (group 1), 10:00-18:00, D00.021/D00.017

Prof. Dr. Heinrich Leonhardt, Dr. Daniela Meilinger

Content: This course teaches how to culture and handle mouse embryonic stem cells and moreover deepens the fundamental understanding of DNA methylation processes in mammalian cells. During the course, a rescue assay of knockout cell lines will be performed as well as a differentiation assay where ES cells will be differentiated into epiblast cells.

Skills Taught: ES cell culture, FACS sorting, genomic DNA extraction, bisulfite treatment, pyrosequencing, differentiation assay, Immunostaining, fluorescence microscopy FRAP, confocal microscopy, image analysis

Recommended Lectures: Epigenetics (SS), Methods in Molecular Biology (WS)


Practical Course: Webtool development for biologists using Python (incl. Seminar)

  • 16.03.-27.03.2019 (group 1), 10:00-18:00, D00.021/D00.017

Prof. Dr. Heinrich Leonhardt, Dr. Sebsatian Bultmann

Content:

Participants in “Webtool development for biologists using python” obtain basic knowledge of the python programming language and its use on developing web tools for life science purposes.

  • Basic introduction into programming/scripting languages
  • Basic knowledge of the Python syntax
  • Data manipulation using the pandas library
  • Web development using the flask.py framework
  • Basic HTML knowledge
  • Basic CSS knowledge
  • Basic Javascript knowledge

A mandatory part of this course is an accompanying seminar, in which the students will acquire the basic Python programming skills which will be applied in the course.

Qualification goals: Students can apply theoretical and practical knowledge to solve problems using python programming. Students will obtain the skills necessary to automate routine tasks (e.g. calculations; data formatting and visualization) and make these available for others as web tools.

This class is supported by DataCamp, the most intuitive learning platform for data science. Learn R, Python and SQL the way you learn best through a combination of short expert videos and hands-on-the-keyboard exercises. Take over 100+ courses by expert instructors on topics such as importing data, data visualization or machine learning and learn faster through immediate and personalised feedback on every exercise.

Practical Course: Lab Methods Course - MHB, MCB  & PS 

  • 20.06.-30.06.2017 (group 1), 10:00-18:00, D00.021/D00.017
  • 10.07.-21.08.2017 (group 2), 10:00-18:00, D00.021/D00.017

Prof. Dr. Heinrich Leonhardt, Dr. Daniela Meilinger, Dr. Dagmar Hann, Dr. Frank Landgraf, 

Content: This course is especially recommended for 1st semester Master Students, as it teaches essential and basic methods in molecular and cellular biology in-depth. Cloning techniques will be explored to generate a GFP Fusion protein including various mutants in order to investigate protein domain functions in vivo. After having successfully cloned different constructs, a Co-Immunoprecipitation will be performed to check for potential protein-protein interactions in mammalian cells. Finally, cells will be transfected with these constructs to visualize localisation in mammalian cells using fluorescence microscopy.

Skills Taught: Cloning to generate a GFP Fusion Protein, primer design, PCR, vector construction, ligation, transformation, "Mini-Prep" and "Maxi-Prep", Transfection of mammalian cells, Western Blot, Co-Immunoprecipitation, Immunostaining, fluorescence microscopy

Recommended Lectures: Methods in Molecular Biology (WS)